By G. L. Nicolson (auth.), James K. Koehler Ph. D. (eds.)
The use of the time period "advanced" within the identify of this publication is a little ar bitrary and intensely a lot relative with admire to time. Many suggestions that have been thought of on the "cutting area" of ultrastructural technique quite a few years in the past at the moment are rou tin ely utilized in various laboratories. you can still cite freeze-fracture, cryothin sectioning, or certainly many of the box of experiment ning electron microscopy as concrete examples. hence using the time period "ad vanced strategies" needs to be interpreted in regards to the current cutting-edge, and comes in handy simply in informing the aptitude reader that this quantity isn't a primer for use as an preliminary advent into easy organic elec tron microscopy. many glorious volumes have stuffed that area of interest long ago few years, and it isn't meant that this modest publication be an entire com pendium of the sphere. in addition, any restricted choice of papers on advanc ed innovations inevitably displays the personal tastes and arbitrary whims of the editor, thereby except for many both vital systems which the a professional reader will without problems establish. the 1st quantity of this sequence seemed nearly 5 years in the past and illustrated options that have been proposal to symbolize complex and but ba sically morphological equipment for gaining elevated ultrastructural informa tion from organic specimens. the current quantity, nevertheless, stresses strategies which offer particular physicochemical info at the speci mens as well as the structural information.
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Extra info for Advanced Techniques in Biological Electron Microscopy II: Specific Ultrastructural Probes
1. , 1975). Several other uses of lectin-ferritin conjugates are listed in Table 4. , 1971). , 150 A) may be important in determining the extent of labeling due to steric considerations; thus, certain types of receptors may not be accessible to labeling. The purity of ferritin is important in the construction of ferritin conjugates, and the conjugates, once synthesized, should be used within two weeks (author's unpublished observations) to alleviate possible aggregation, which has been reported to be a problem with some lectin-ferritin conjugates.
HUET, M. -P. REVEL, and]. ROTH are gratefully acknowledged. , Heaysman, J. E. , Pegrum, S. : Locomotion of fibroblasts in culture. V. Surface marking with concanavalin A. Exptl. Cell Res. 73,536 - 539 (1972) Ackerman, G. , Waksal, S. : Ultrastructural localization of concanavalin A binding sites on the surface of differentiating hemopoietic cells. Cell Tiss. Res. 150, 331- 342 (1974) Agrawal, B. B. , Goldstein, 1. : Protein-carbohydrate interaction. VI. Isolation of concanavalin A by specific adsorption on cross-linked dextran gels.
C. HUET) X 14,000 24 ·G. L. NICOLSON postincubated at 37° C, lectin-induced redistribution occurred (Fig. 5 b, c), eventually leading to endocytosis of lectin-peroxidase complexes by 60 min (Fig. 5 d). When HUETand BERNHARD (1974) examined an SV 40-transformed hamster embryo cell line (Cl 2TSVs), these events occurred even more rapidly than on the untransformed cell surface (Fig. 5 e - g), indicating a difference in the rate of mobiliry and endocytosis of Con A receptors on the transformed cell line.