Download Amyloid Precursor Protein: A Practical Approach by Weiming Xia, Huaxi Xu PDF

By Weiming Xia, Huaxi Xu

ISBN-10: 0849322456

ISBN-13: 9780849322457

Within the look for a good remedy for Alzheimer's ailment, APP is a distinct version protein that illustrates the big variety of simple and complex characterization strategies on hand. Exploring various organic suggestions to elucidate the constitution and serve as of this transmembrane protein, this article offers every one process with targeted, step by step protocols to accomplish reproducible effects and supply a framework for learning different membrane proteins.

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Place the cells in 2 ml of prewarmed medium and incubate at 37˚C for various periods. 6. 5. Incubate the cells for another 5 min. Collect the supernatant, repeat the acidic wash to remove residual surface-bound antibody and pool both supernatants. The acid wash detaches 90 to 95% of cell surface-bound antibody 7. 2 N NaOH. 8. Measure the radioactivity of all fractions in a gamma counter. To calculate the specific binding, the radioactivity from untransfected control cells is subtracted from the counts obtained from the transfected cells in each condition.

270, 4205, 1995. 13. Murayama, Y. et al. Cell surface receptor function of amyloid precursor protein that activates Ser/Thr kinases. Gerontology 42, 2, 1996. 14. Zheng, H. et al. beta-Amyloid precursor protein-deficient mice show reactive gliosis and decreased locomotor activity. Cell 81, 525, 1995. 15. Hendriks, L. and Van Broeckhoven, C. A beta A4 amyloid precursor protein gene and Alzheimer’s disease. Eur. J. Biochem. 237, 6, 1996. 16. P. Cellular actions of beta-amyloid precursor protein and its soluble and fibrillogenic derivatives.

28. Iwasaki, W. et al. Solution structure of midkine, a new heparin-binding growth factor. EMBO J. 16, 6936, 1997. 29. Zhou, H. et al. The solution structure of the N-terminal domain of hepatocyte growth factor reveals a potential heparin-binding site. Structure 6, 109, 1998. 30. J. et al. Solution structure of the heparin-binding domain of vascular endothelial growth factor. Structure 6, 637, 1998. 31. U. et al. From differentiation to proliferation: the secretory amyloid precursor protein as a local mediator of growth in thyroid epithelial cells.

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